Supercritical Fluid Extraction from Zataria multiflora Boiss and Impregnation of Bioactive Compounds in PLA for the Development of Materials with Antibacterial Properties

In this research, the extraction with supercritical carbon dioxide (SC-CO2) and subsequent impregnation of extracted bioactive compounds from Zataria multiflora Boiss (Z. multiflora) into polylactic acid (PLA) films was investigated. The effects temperature (318 338 K), pressure (15 25 MPa) cosolvent presence (0 3 mol%) on yield were studied. SC-CO2 assisted runs carried out in a discontinuous mode at different MPa), 328 time (2 8 h) values, using 0.5 MPa min?1 as constant value depressurization rate. ANOVA results confirmed that pressure, temperature, influenced yield. Moreover, antioxidant activities extracts Z. evaluated 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. addition, antibacterial screened against standard strains Staphylococcus aureus (S. aureus) Escherichia coli (E. coli). investigation indicated obtained aerial parts possessed properties. impregnated samples presented strong activity selected microorganisms.